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Detection Image Detection method details

Name:

 QT-TAX-GM-007

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene

Comment:

 target is the endogenous lectin (Le1) gene of soybean

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 taxon-specific

Target Species name:

 Glycine max

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Le1n02-5'

  • Sequence:

    GCCCTCTACTCCACCCCCA

  • Size:

    19

  • Reverse Primer

  • Name:

    Le1n02-3'

  • Sequence:

    GCCCATCTGCAAGCCTTTTT

  • Size:

    20

  • Probe

  • Name:

    Le1-Taq

  • Sequence:

    AGCTTCGCCGCTTCCTTCAACTTCAC

  • Size:

    26

  • Amplicon:

  • Sequence:

    gccctctactccacccccannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagcttcgccgcttccttcaacttcacnnnnnnnnnnnnnnnnnnaaaaaggcttgcagatgggc

  • Size:

    118

Documents

Citation Type Local copy
Shindo, Y., Kuribara, H., Matsuoka, T., Futo, S., Sawada, C., Shono, J., Akiyama, H., Goda, Y., Toyoda, M., Hino, A. (2002). Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules. Journal of the AOAC International, 85, 1119-1126Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QT-TAX-GM-007Link to the document url. EU reference method protocol